Bit · Biochem

Vitamin Deficiencies — Water-Soluble vs Fat-Soluble

Thirteen vitamins, thirteen deficiency syndromes. Water-soluble vitamins wash out and rarely store; fat-soluble vitamins do the opposite.

Mechanism

Water-soluble (B-complex, C) are not stored substantially — deficiency develops over weeks. Fat-soluble (A, D, E, K) are stored in liver and adipose — deficiency develops over months, but toxicity is more common.

Differentiator Table

VitaminFunctionDeficiency syndromeDistinctive clue
A (retinol)Vision pigments, epithelial maintenanceNight blindness, xerophthalmia, Bitot spots, immune dysfunctionSquamous metaplasia
DCa²⁺ + PO₄³⁻ regulationRickets (children), osteomalacia (adults)Bowed legs, looser zones, low Ca/PO₄
E (tocopherol)AntioxidantHemolytic anemia, ataxia, peripheral neuropathyMimics B12 minus megaloblastic
Kγ-carboxylation of clotting factors 2,7,9,10,C,SBleeding, prolonged PT (then PTT)Newborn hemorrhagic disease; warfarin reverses K
B1 (thiamine)α-ketoacid dehydrogenases, transketolaseWet beriberi (high-output HF) / dry beriberi (neuropathy); Wernicke-KorsakoffAlcoholic with confusion-ataxia-ophthalmoplegia
B2 (riboflavin)FAD/FMNCheilosis, corneal vascularization'2 C's of B2'
B3 (niacin)NAD/NADPPellagra — 3 D's: Dermatitis, Diarrhea, DementiaCasal necklace; tryptophan source
B5 (pantothenate)Coenzyme ADermatitis, enteritis, alopecia, adrenal insufficiencyRare
B6 (pyridoxine)Decarboxylation of AAs, heme synthesisSideroblastic anemia, peripheral neuropathy, convulsionsINH → B6 deficiency seizures
B7 (biotin)CarboxylationDermatitis, alopecia, enteritisRaw egg whites (avidin)
B9 (folate)DNA synthesis (methylation)Megaloblastic anemia WITHOUT neuro signs; neural tube defectsPregnancy; methotrexate
B12 (cobalamin)Homocysteine→methionine; methylmalonyl-CoA→succinylMegaloblastic anemia WITH neuro (subacute combined degeneration); ↑ MMAVegans, terminal ileum disease, pernicious anemia
C (ascorbate)Hydroxylation of collagen Pro/Lys; antioxidantScurvy — bleeding gums, perifollicular hemorrhages, poor wound healingSailors, infants on cow's milk

The Pivot

Two splits do most of the work:

  1. Megaloblastic anemia — neuro symptoms? Yes → B12. No → folate.
  2. Bleeding — PT/PTT prolonged after newborn period? → vitamin K. Bleeding gums, easy bruising in a teenager who only eats white rice → vitamin C.

NBME-Style Stem

A 64-year-old woman presents with progressive numbness, gait instability, and forgetfulness. Hemoglobin is 9.2 g/dL with MCV 116 fL. Hypersegmented neutrophils are seen on peripheral smear. Serum methylmalonic acid and homocysteine are both elevated. Which of the following is the most likely diagnosis?
Concept Anchor
Each vitamin maps to a specific enzyme (or set of enzymes); deficiency manifests as the failure of those enzymes' work. The high-yield trick is the B12/folate split — only B12 deficiency causes neuro deficits and elevated MMA.

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